Examination of the Accuracy of the Quantitative of Kit for Determination of Zinc Concentration in Seminal Fluid

Introduction: The role of zinc is critical to reproduction potential. Seminal zinc is thought to be derived almost exclusively from prostatic secretions. Sperm motility is significantly influenced by zinc. Zinc deficiency has been linked with male sterility and subfertility. Materials and Methods: The semen samples were obtained from 300 male partners of infertile couples who attended the Department Biomedical and Genetics of the Hanoi medical university within the age 18-50 years and semen samples were analyzed for the routine seminal parameters. Routine semen analysis was performed according to WHO 2010 guidelines. After the semen analysis, samples were centrifuged at 1500 x g for 10min and zinc and fructose concentrations assayed from the supernatant (i.e. seminal plasma). Zinc concentration was assessed using spectrophotometry (5- Br- PAPS method) - direct colorimetric test without deproteinization of the sample. At pH 8.6, in a buffered media, zinc reacts with specific complexing 5-Br-PAPS form a stable color compound. Results: Linear regression model: y = 0.0666x + 1.2026 with correlation coefficient r = 0.9956. The calibration function: y = 0.9977x with R2 = 0.9995. Repeatability: SD = 0.004 and coefficient of variation CV% = 0.27%

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The authors declare no conflict of interest.

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The datasets used in this study are openly available at [repository link] and the source code is available on GitHub at [GitHub link].

This work did not receive any external funding.