Development and validation of a New HPLC Method for Determination of Clorazepate Dipotassium in Capsule Formulations

London Journal of Research in Science: Natural and Formal
Volume | Issue | Compilation
Authored by Safwan Ashour , Nuha Kattan
Classification: For Code: 030499, 030405
Keywords: clorazepate dipotassium, high performance liquid chromatography, capsules.
Language: English

A simple, accurate and rapid high performance liquid chromatographic method has been developed and applied for determination of clorazepate dipotassium in capsules. Rosuvastatin calcium was used as internal standard. The determination was performed on Supelcosil C8 DB column (250×4.6mm i.d., 5µm particle size) at 25 °C using a mobile phase composed of MeOH and 0.1 M HCOOH pH 2.16 (67:33, v/v), pumped at a flow rate 1.0 mL min-1. The photodiode array detector was operated at 245 nm. The retention times for rosuvastatin and clorazepate were about 5.05 and 6.85 min, respectively. Results of assay were statistically evaluated for its accuracy and precision. Linearity range was 2.15–400.0 µg mL-1. The limits of quantification and detection were 1.31 and 0.39 µg mL-1, respectively. The intra-day precision results, expressed by relative standard deviation values, were lower than 2.35%. The validated method has been successfully applied to the analysis of clorazepate dipotassium in capsules with no interference from the excipients.



author

For Authors

Author Membership provide access to scientific innovation, next generation tools, access to conferences/seminars
/symposiums/webinars, networking opportunities, and privileged benefits.
Authors may submit research manuscript or paper without being an existing member of LJP. Once a non-member author submits a research paper he/she becomes a part of "Provisional Author Membership".

Know more

institutes

For Institutions

Society flourish when two institutions come together." Organizations, research institutes, and universities can join LJP Subscription membership or privileged "Fellow Membership" membership facilitating researchers to publish their work with us, become peer reviewers and join us on Advisory Board.

Know more

subsribe

For Subscribers

Subscribe to distinguished STM (scientific, technical, and medical) publisher. Subscription membership is available for individuals universities and institutions (print & online). Subscribers can access journals from our libraries, published in different formats like Printed Hardcopy, Interactive PDFs, EPUBs, eBooks, indexable documents and the author managed dynamic live web page articles, LaTeX, PDFs etc.

Know more