Efficacy of Different Soil Amendments and Bio-Agents Against Fusarium Oxysporum f. sp. Lentis in Vitro & in Vivo

Five organic amendments were evaluated against wilt of lentil which were found more or less effective. Maximum disease control (42.85%) found in neem cake followed by mustard cake (38.23%), parthenium (32.90%), linseed cake (28.57%) and sawdust was least in reducing wilt (23.66%) in 2016- 17. Similar results were also observed in the year 2017-18.Inhibitory effect of bioagents was tested against Fusarium oxysporum f. sp. lentis in vitro. Maximum(65.94%) mycelial growth was inhibited by Pseudomonas fluorescens followed by Bacillus subtilis (62.23%), T. viride (39.62%)and T. virense (39.22%).T. harzianum was found least effective inhibition of mycelia growth (35.65%) in dual plate technique. All five bio-agents evaluated against F. o. f. sp. lentis in vitro were also tested in vivo conditions, where they also were found effective in wilt management. Maximum disease control (42.10%) was recorded with P. fluorescens@ 10 g/kg seed followed by Bacillus subtilis (38.27%) @ 10 g/kg seed, T. viride (32.70%) @ 4 g/kg seed and T. virens (32.80%) @ 4 g/kg seed . T. harzianum was least effective in reducing wilt incidence (30.40%) @ 4 g/kg seed in 2016-17. Similar results were also observed in the year 2017-18. Disease incidence was maximum at 90 days after sowing as compared to 60 and 30 days after sowing in both the years.

The authors declare no conflict of interest.

Not applicable

The datasets used in this study are openly available at [repository link] and the source code is available on GitHub at [GitHub link].

This work did not receive any external funding.