Production of Protein Hydrolysate and Biodiesel from Black Soldier Fly larva Cultivated using Rotten Avocado and Tofu Residue

London Journal of Research in Science: Natural and Formal
Volume | Issue | Compilation
Authored by M. Yusuf Abduh , Felicia Tejo , Ghassani Muzakki Sabila Hidaya , Romario Joseph, Ramadhani Eka Putra , Robert Manurung, Agus Dana Permana ,& Miranti Indar Mandasari
Classification: NA
Keywords: amino acid, biodiesel, fatty acid, Hermetia illucens, protein hydrolysate.
Language: English

The larva of Hermetia illucens L. (black soldier fly larva) can convert various types of organic to produce protein and lipid rich biomass which can be further valorized to produce  valuable bioproducts. This study was carried out to investigate the potential application of tofu residue and rotten avocado as substrates for the cultivation of black soldier fly larvae as an alternative feedstock to produce protein hydrolysate and biodiesel. The larvae were cultivated for 24 days in a screen house (temperature range of 19–30 ° C, relative humidity of 35–95%, and light intensity of 0.03–20 W/m 2 ). The larvae were fed with tofu residue and rotten avocado once every three days. After 24 days of cultivation, the larvae were harvested with a biomass weight of 233-267 mg/larvae and larval length of 2.2-2.3 cm. The harvested biomass had total protein and lipid content in the range of 21.2-47.2% and 20.8-28.9%, respectively. Lipid isolated from the harvested biomass was reacted with methanol and lipase to produce biodiesel which mainly composed of fatty acid methyl laureate (19.2-28.7%), fatty acid methyl oleate (22.1-23.7%), and fatty acid methyl linoleate (15.4-:21.1%). The ester content, acid value and iodine number of the produced biodiesel were also determined. The defatted biomass was hydrolyzed with 5% bromelain enzyme to produce protein hydrolysate with the degree of hydrolysis and productivity of the protein hydrolysate lies in the range of 46.7-52.6% and 0.02-0.04 g/ml/batch. Amino acid in the protein hydrolysate was determined and mainly composed of glutamic acid (16.5-18.2%), alanine (16.7-18.1%), lysine (9.1-
9.4%) and valine (7.9-8.5%).

               

Moved Temporarily

The document has moved here.


author

For Authors

Author Membership provide access to scientific innovation, next generation tools, access to conferences/seminars
/symposiums/webinars, networking opportunities, and privileged benefits.
Authors may submit research manuscript or paper without being an existing member of LJP. Once a non-member author submits a research paper he/she becomes a part of "Provisional Author Membership".

Know more

institutes

For Institutions

Society flourish when two institutions come together." Organizations, research institutes, and universities can join LJP Subscription membership or privileged "Fellow Membership" membership facilitating researchers to publish their work with us, become peer reviewers and join us on Advisory Board.

Know more

subsribe

For Subscribers

Subscribe to distinguished STM (scientific, technical, and medical) publisher. Subscription membership is available for individuals universities and institutions (print & online). Subscribers can access journals from our libraries, published in different formats like Printed Hardcopy, Interactive PDFs, EPUBs, eBooks, indexable documents and the author managed dynamic live web page articles, LaTeX, PDFs etc.

Know more