Abstract
Chinese evergreen (Aglaonema commutatum) is being cultivated recently as a decorative plant in Egypt. In the present study trials were done to micropropagate it in vitro. For this purpose nodal segments were excised from shoots and cultured on Murashige and Skoog’s (MS) culture media augmented with naphthalene acetic acid (NAA) 0.1 and 0.5 mg/l in combination with thiadiozhuros (TDZ) O.5 , 1.0 and 2 mg/l, followed by transfer to hormone ??free culture media added to it active coal, for four weeks and regularly subcultured four successive times . Results of the present study have shown that 0.5 mg/l NAA + 2 mg/l TDZ for eight weeks were superior to the other hormonal combinations used. This treatment led to significant increases over the other treatments in number of shoots obtained per explant, the number of leaves per shoot and number of roots per plantlets 4.67?ñ0.58, 2?ñ0.00, 7.00?ñ1.00 respectively, the average length of shoot and roots 8.50?ñ0.10, 7.33?ñ0.15 cm respectively, and the average fresh weight per regenerant 2 9.99?ñ0.22 gram with a corresponding dry weight 0.30?ñ0.01 gram. The obtained regenerates were easily acclimatized and transferred to pots. The obtained results may facilitate production of A. cammutatum on the commercial level in our country.
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