Expression of Selectins in the Development of Diabetic Nephropathy in Patients with type 2 Diabetes

Research objective: The work is devoted to studying the role of the expression of P-selectin (CD62P) and E-selectin(CD62E) in the glomerular apillary endothelium and peritubular capillaries and their role in the development and progression of morphological changes in renal tissue during diabetic nephropathy in patients with type 2 diabetes mellitus.
Materialandmethods: The examination was carried out in 50 patients with type 2 diabetes mellitus (average age 66.58±3.27 years). All patients underwent light and immunofluorescence microscopy of renal tissue biopsies obtained by intravita percutaneous kidney biopsies. Morphological changes in tissue were assessed in accordance with the latest international classification of diabetic nephropathy, developed in 2010. According to light microscopy, class IIa (mild mesangial expansion) was identified in 12 patients, class IIb (severe mesangial expansion) was identified in 14 patients. , in 19 patients – class III (nodular Kimmelstiel-Wilson lesions) and in 5 patients – class IV (advanced diabetic glomerulosclerosis). The expression of P- and E-selectin was determined using monoclonal antibodies labeled with FITC (anti-human CD62P (P-Selectin) Antibody and anti-human CD62E (E-Selectin) Antibody) (USA). The intensity of expression in points (0–4), the nature and location of selectin expression in the glomerular endothelium and in peritubular capillaries were assessed.

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The authors declare no conflict of interest.

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The datasets used in this study are openly available at [repository link] and the source code is available on GitHub at [GitHub link].

This work did not receive any external funding.