Anti-Proliferative Activity of Ethyl Acetate Leaf Extract of Annona Muricata L. on Selected Carcinoma Human Cell Lines

Medicinal plants are a rich source of active phytochemicals and have been in use in ethnomedicine. This study investigates the anti-proliferative activity of ethyl acetate leaf extract of Annona muricata L. on selected carcinoma human cell lines of MCF 7, HT 29, HT 116 and C4-2WT. MTT (3- (4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, methylene blue, Trypan Blue exclusion assay and wound or scratch assays were carried out to evaluate, in vitro, the cytotoxicity potential of the ethyl acetate leaf extract of Annona muricata L. Results showed that anti-proliferation activity increased with increase in the concentration of the extract used. The minimum concentration of the extract required for 50% inhibition (GI50) of the different cell lines calculated after MTT test were as follows: MCF-7 = 2.61 ?æg/ml, C4-2WT = 9.33 ?æg/ml, HCT 29 = 4.97 ?æg/ml and HCT 116 =2.13 ?æg/ml. There was a significant reduction (p

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The authors declare no conflict of interest.

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The datasets used in this study are openly available at [repository link] and the source code is available on GitHub at [GitHub link].

This work did not receive any external funding.